IKMC Project Report - KOMP-CSD (ID: 48890)

H2-Eb1 MGI:95901 ENSMUSG00000060586 OTTMUSG00000037123
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000074557 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MVWLPRVPCVAAVILLLTVLSPPVALVRDSRPWFLEYCKSECHFYNGTQRVRLLERYFYNLEENLRFDSDVGEFRAVTEL
GRPDAENWNSQPEFLEQKRAEVDTVCRHNYEISDKFLVRRR

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000358685 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000657636 MHC_II_b_N (74/74 aa) CDS CDS + stop codon + UTR
ENSMUSE00000657635 Ig_C1-set (81/81 aa) CDS Deleted
ENSMUSE00000657634 CDS Deleted
ENSMUSE00000486837 CDS Deleted
ENSMUSE00000698442 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available