IKMC Project Report - NorCOMM (ID: 49079)

Ccl5 MGI:98262 ENSMUSG00000035042 OTTMUSG00000000992
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Targeting Confirmed

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). This mutation is of type 'Deletion' (more information on IKMC alleles can be found here). The table below shows the predicted structure of the gene transcripts. Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000035938 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MKISAAALTIILTAAALCTPAPASP

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000277128 UTR + start codon + CDS
ENSMUSE00000277119 Chemokine_IL8-like_dom (34/60 aa) CDS Deleted
ENSMUSE00000397300 Chemokine_IL8-like_dom (27/60 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000125015 retained_intron No analysis available: incomplete transcript
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ES Cell Clones (Conditional Ready)

Note: These mutations can be made conditional by a "docking" procedure which uses the attP/puro docking site in the cassette.

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order N01644P1_C_249W_B7 N01644P1_T_169.62_B02 Deletion (Promotor Driven Cassette) C2 view no no data reported    ( about )
order N01644P1_C_249W_C6 N01644P1_T_169.62_B02 Deletion (Promotor Driven Cassette) C2 view no no data reported    ( about )
order N01644P1_C_249W_C7 N01644P1_T_169.62_B02 Deletion (Promotor Driven Cassette) C2 view no no data reported    ( about )
order N01644P1_C_249W_D5 N01644P1_T_169.62_B02 Deletion (Promotor Driven Cassette) C2 view no no data reported    ( about )
order N01644P1_C_249W_E5 N01644P1_T_169.62_B02 Deletion (Promotor Driven Cassette) C2 view no no data reported    ( about )
order N01644P1_C_249W_F5 N01644P1_T_169.62_B02 Deletion (Promotor Driven Cassette) C2 view no no data reported    ( about )
order N01644P1_C_249W_F6 N01644P1_T_169.62_B02 Deletion (Promotor Driven Cassette) C2 view no no data reported    ( about )
order N01644P1_C_249W_H7 N01644P1_T_169.62_B02 Deletion (Promotor Driven Cassette) C2 view no no data reported    ( about )
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 230949 Deletion (Promotor Driven Cassette) N01644P1_T_169.62_B02 L1L2_GOHANU L3L4_pZero_DTA_kan view

Intermediate Vectors

Design ID Vector Type Intermediate Vector
230949 Deletion N01644P1_I_169.62_B02