IKMC Project Report - KOMP-CSD (ID: 49252)

Snap25 MGI:98331 ENSMUSG00000027273 OTTMUSG00000015556
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 3 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000028727 protein_coding No protein product (NMD) view

Predicted translation:

MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEENNWNALRKGWTKSIRT*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000816437 UTR UTR
ENSMUSE00001008353 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000167774 CDS CDS
ENSMUSE00000167770 CDS Deleted
ENSMUSE00000167772 SNAP-25 (3/51 aa) CDS CDS + stop codon + UTR
ENSMUSE00000167776 SNAP-25 (43/51 aa) CDS
ENSMUSE00000404871 T_SNARE_dom (39/59 aa) | SNAP-25 (7/51 aa) CDS
ENSMUSE00000706204 T_SNARE_dom (20/59 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000110098 protein_coding No protein product (NMD) view

Predicted translation:

MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEENNSIVSKKA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000706206 UTR UTR
ENSMUSE00001008353 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000167774 CDS CDS
ENSMUSE00000167770 CDS Deleted
ENSMUSE00000682755 SNAP-25 (3/51 aa) CDS CDS + stop codon + UTR
ENSMUSE00000167776 SNAP-25 (43/51 aa) CDS
ENSMUSE00000404871 T_SNARE_dom (39/59 aa) | SNAP-25 (7/51 aa) CDS
ENSMUSE00000814357 T_SNARE_dom (20/59 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000125486 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available