IKMC Project Report - EUCOMM (ID: 49445)

F2r MGI:101802 ENSMUSG00000048376
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Targeting Confirmed

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000059193 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MGPRRLLIVALGLSLCGPLLSSRVPMSQP

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000353700 UTR + start codon + CDS
ENSMUSE00000340298 7TM_GPCR_Rhodpsn (252/252 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted

ES Cell Clones With Knockout First Mutation (Reporter Tagged Insertion With Conditional Potential)

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool - LRPCR Genotyping Primers
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order EPD0236_1_G10 PRPGS00092_C_B11 F2rtm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) JM8.N4 view no view    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 87372 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) PG00092_W_4_B11 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 87372 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) PG00092_W_1_B11 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 87372 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) PG00092_W_3_B11 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 87372 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) PG00092_W_2_B11 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 87372 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) PRPGS00092_C_B11 L1L2_Bact_P L3L4_pD223_DTA_spec view
show/hide more targeting vectors

Intermediate Vectors

Design ID Vector Type Intermediate Vector
87372 Knockout First, Reporter-tagged insertion with conditional potential PCS00092_A_B11
87372 Knockout First, Reporter-tagged insertion with conditional potential PCS00092_C_B11