IKMC Project Report - KOMP-CSD (ID: 66376)

0610040J01Rik MGI:1923511 ENSMUSG00000060512
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000081747 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MGCRCCKMIQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000515238 UTR UTR
ENSMUSE00000514232 UTR UTR
ENSMUSE00000257985 UTR + start codon + CDS
ENSMUSE00000391744 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 113561 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00118_X_1_F05 L1L2_Bact_P L3L4_pZero_DTA_kan view
order 113561 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00118_X_4_F05 L1L2_Bact_P L3L4_pZero_DTA_kan view
order 113561 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PRPGS00118_B_F05 L1L2_Bact_P L3L4_pZero_DTA_kan view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
113561 Knockout-First - Reporter Tagged Insertion PCS00118_A_F05
113561 Knockout-First - Reporter Tagged Insertion PCS00118_B_F05