IKMC Project Report - EUCOMM (ID: 71564)

Park7 MGI:2135637 ENSMUSG00000028964 OTTMUSG00000010158
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 10 wild type transcripts, of which 8 are protein-coding. Following removal of the floxed region, 8 transcripts are predicted to produce a truncated protein product of which 4 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000030805 protein_coding No protein product (NMD) view

Predicted translation:

MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVMICPDTSLEDAKTQVLRLCWLTK*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000747675 UTR UTR
ENSMUSE00000984201 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001072943 ThiJ/PfpI (32/141 aa) CDS CDS
ENSMUSE00000995971 ThiJ/PfpI (20/141 aa) CDS Deleted
ENSMUSE00000183899 ThiJ/PfpI (24/141 aa) CDS Deleted
ENSMUSE00001087416 ThiJ/PfpI (30/141 aa) CDS CDS + stop codon + UTR
ENSMUSE00000667059 ThiJ/PfpI (37/141 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000105674 protein_coding No protein product (NMD) view

Predicted translation:

MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVMICPDTSLEDAKTQVLRLCWLTK*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000667058 UTR UTR
ENSMUSE00000667057 UTR UTR
ENSMUSE00000984201 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001072943 ThiJ/PfpI (32/141 aa) CDS CDS
ENSMUSE00000995971 ThiJ/PfpI (20/141 aa) CDS Deleted
ENSMUSE00000183899 ThiJ/PfpI (24/141 aa) CDS Deleted
ENSMUSE00001087416 ThiJ/PfpI (30/141 aa) CDS CDS + stop codon + UTR
ENSMUSE00000667056 ThiJ/PfpI (37/141 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000105673 protein_coding No protein product (NMD) view

Predicted translation:

MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVMICPDTSLEDAKTQVLRLCWLTK*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000667055 UTR UTR
ENSMUSE00000984201 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001072943 ThiJ/PfpI (32/141 aa) CDS CDS
ENSMUSE00000995971 ThiJ/PfpI (20/141 aa) CDS Deleted
ENSMUSE00000183899 ThiJ/PfpI (24/141 aa) CDS Deleted
ENSMUSE00001087416 ThiJ/PfpI (30/141 aa) CDS CDS + stop codon + UTR
ENSMUSE00000667054 ThiJ/PfpI (37/141 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000105675 protein_coding No protein product (NMD) view

Predicted translation:

MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVMICPDTSLEDAKTQVLRLCWLTK*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000667060 UTR UTR
ENSMUSE00000984201 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001072943 ThiJ/PfpI (32/141 aa) CDS CDS
ENSMUSE00000995971 ThiJ/PfpI (20/141 aa) CDS Deleted
ENSMUSE00000183899 ThiJ/PfpI (24/141 aa) CDS Deleted
ENSMUSE00001087416 ThiJ/PfpI (30/141 aa) CDS CDS + stop codon + UTR
ENSMUSE00000667059 ThiJ/PfpI (37/141 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000105676 protein_coding No analysis available: incomplete transcript
ENSMUST00000128075 protein_coding No analysis available: incomplete transcript
ENSMUST00000134751 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVMICPDTSLEDAKTQ

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000734849 UTR UTR
ENSMUSE00000984201 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001072943 ThiJ/PfpI (32/61 aa) CDS CDS
ENSMUSE00000815633 ThiJ/PfpI (29/61 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000146184 protein_coding No analysis available: incomplete transcript
ENSMUST00000148626 retained_intron No analysis available: incomplete transcript
ENSMUST00000132265 processed_transcript Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available