IKMC Project Report - EUCOMM (ID: 71986)

Oat MGI:97394 ENSMUSG00000030934
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000084500 protein_coding No protein product (NMD) view

Predicted translation:

MLSKLASLQTIAALRRGVHTSVASATSVATKKTEQGPPSSEYIFERESKYGAHNYHPLPVALERGKGIYMWDVEGRQYFD
FLSAYGAVSQGHCHPKIIDAMKSQVDKLTLTSRAFYNNVLGEYEEYITKLFNYNKVLPMNTGVEAGETACKLARRWGYTV
KGIQKYKAKIVFAACSSGSKCCCLHGGAHPG*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000407701 UTR UTR
ENSMUSE00000516428 Aminotrans_3 (5/322 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000300591 Aminotrans_3 (76/322 aa) CDS CDS
ENSMUSE00000204918 Aminotrans_3 (33/322 aa) CDS CDS
ENSMUSE00000300578 Aminotrans_3 (43/322 aa) CDS Deleted
ENSMUSE00000300570 Aminotrans_3 (41/322 aa) CDS CDS + stop codon + UTR
ENSMUSE00000204903 Aminotrans_3 (43/322 aa) CDS
ENSMUSE00000204900 Aminotrans_3 (38/322 aa) CDS
ENSMUSE00000204916 Aminotrans_3 (46/322 aa) CDS
ENSMUSE00000526592 CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available