IKMC Project Report - EUCOMM (ID: 75388)

Donson MGI:1890621 ENSMUSG00000022960 OTTMUSG00000019888
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 10 wild type transcripts, of which 5 are protein-coding. Following removal of the floxed region, 5 transcripts are predicted to produce a truncated protein product of which 3 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000023682 protein_coding No protein product (NMD) view

Predicted translation: 

MAVSVPGYSPSFKRPPETVRLRRKRSRDHGAAVPASLPEPAPRRAALAAGLPLRPFPTAGGRGGAAATIARRNPFARLDN
RPRVSDEASEEPLRGPQGASGPLLDSNEENNLLWEDTSSHERTGTELSQSQRVSLSESDTWSSDGTELPVDWSIKTRLLF
TSSQPFSWADHLKAQEEAQGLVQHCRATEVTLPQSIQVLNFLCLY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000983185 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001049325 CDS CDS
ENSMUSE00000968847 CDS CDS
ENSMUSE00001058002 CDS Deleted
ENSMUSE00001071387 CDS Deleted
ENSMUSE00001074867 CDS CDS + stop codon + UTR
ENSMUSE00001064426 CDS
ENSMUSE00001023438 CDS
ENSMUSE00001041358 CDS
ENSMUSE00001044460 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000117159 protein_coding No protein product (NMD) view

Predicted translation: 

MAVSVPGYSPSFKRPPETVRLRRKRSRDHGAAVPASLPEPAPRRAALAAGLPLRPFPTAGGRGGAAATIARRNPFARLDN
RPRVSDEASEEPLRGPQGASGPLLDSNEENNLLWEDTSSHERTGTELSQSQRVSLSESDTWSSDGTELPVDWSIKTRLLF
TSSQPFSWADHLKAQEEAQGLVQHCRATEVTLPQSIQVLNFLCLY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000983185 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001049325 CDS CDS
ENSMUSE00000968847 CDS CDS
ENSMUSE00001058002 CDS Deleted
ENSMUSE00001071387 CDS Deleted
ENSMUSE00001074867 CDS CDS + stop codon + UTR
ENSMUSE00001064426 CDS
ENSMUSE00001023438 CDS
ENSMUSE00000717644 CDS
ENSMUSE00001047813 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000114031 protein_coding No protein product (NMD) view

Predicted translation: 

MAVSVPGYSPSFKRPPETVRLRRKRSRDHGAAVPASLPEPAPRRAALAAGLPLRPFPTAGGRGGAAATIARRNPFARLDN
RPRVSDEASEEPLRGPQGASGPLLDSNEENNLLWEDTSSHERTGTELSQSQRVSLSESDTWSSDGTELPVDWSIKTRLLF
TSSQPFSWADHLKAQEEAQGLVQHCRATEVTLPQSIQVLNFLCLY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000983185 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001049325 CDS CDS
ENSMUSE00000968847 CDS CDS
ENSMUSE00001058002 CDS Deleted
ENSMUSE00001071387 CDS Deleted
ENSMUSE00001074867 CDS CDS + stop codon + UTR
ENSMUSE00001064426 CDS
ENSMUSE00001023438 CDS
ENSMUSE00001044460 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000138560 protein_coding No analysis available: incomplete transcript
ENSMUST00000133942 protein_coding No analysis available: incomplete transcript
ENSMUST00000139324 nonsense_mediated_decay No protein product (NMD) view

Predicted translation: 

MAVSVPGYSPSFKRPPETVRLRRKRSRDHGAAVPASLPEPAPRRAALAAGLPLRPFPTAGGRGGAAATIARRNPFARLDN
RPRVSDEASEEPLRGPQGASGPLLDSNEENNLLWEDTSSHERTGTELSQSQRVSLSESDTWSSDGTELPVDWSIKTRLLF
TSSQPFSWADHLKAQEEAQGLVQHCRATEVTLPQSIQVLNFLCLY*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000983185 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001049325 CDS CDS
ENSMUSE00000968847 CDS CDS
ENSMUSE00001066946 CDS + stop codon + UTR Deleted
ENSMUSE00001052133 UTR CDS + stop codon + UTR
ENSMUSE00000968312 UTR UTR
ENSMUSE00000994099 UTR UTR
ENSMUSE00000975883 UTR UTR
ENSMUSE00001032795 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000144461 nonsense_mediated_decay No protein product (NMD) view

Predicted translation: 

MAVSVPGYSPSFKRPPETVRLRRKRSRDHGAAVPASLPEPAPRRAALAAGLPLRPFPTAGGRGGAAATIARRNPFARLDN
RPRVSDEASEEPLRGPQGASGPLLDSNEENNLLWEDTSSHERTGTELSQII*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000697839 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001049325 CDS CDS
ENSMUSE00000831688 CDS + stop codon + UTR CDS + stop codon + UTR
ENSMUSE00001031014 UTR Deleted
ENSMUSE00001052133 UTR UTR
ENSMUSE00000968312 UTR UTR
ENSMUSE00000994099 UTR UTR
ENSMUSE00000975883 UTR UTR
ENSMUSE00000775219 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000145833 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000136699 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000123893 retained_intron No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available