IKMC Project Report - EUCOMM (ID: 75473)

Timmdc1 MGI:1922139 ENSMUSG00000002846 OTTMUSG00000028158
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000002925 protein_coding No protein product (NMD) view

Predicted translation:

MGAPPPAPRSRLCGAWGPFPRVFAAGAVAADSPGFVEDREQRSGVSDPGSLESGWDRLRQLFAKDKVHTVLPQGASSGMA
GAGVGELQFS*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000798821 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000982019 Tim17/Tim22/Tim23/PMP24 (43/126 aa) CDS Deleted
ENSMUSE00000130568 Tim17/Tim22/Tim23/PMP24 (30/126 aa) CDS CDS + stop codon + UTR
ENSMUSE00000130579 Tim17/Tim22/Tim23/PMP24 (24/126 aa) CDS
ENSMUSE00000319281 Tim17/Tim22/Tim23/PMP24 (27/126 aa) CDS
ENSMUSE00000130574 Tim17/Tim22/Tim23/PMP24 (5/126 aa) CDS
ENSMUSE00000700399 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000147543 processed_transcript No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors

Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 46124 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) PG00062_A_3_F04 L1L2_gt0 L3L4_pZero_DTA_kan view
order 46124 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) PG00062_A_2_F04 L1L2_gt0 L3L4_pZero_DTA_kan view
order 46124 Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) PG00062_A_1_F04 L1L2_gt0 L3L4_pZero_DTA_kan view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
46124 Knockout First, Reporter-tagged insertion with conditional potential PCS00062_A_F03