IKMC Project Report - KOMP-CSD (ID: 75511)

Nuak1 MGI:1925226 ENSMUSG00000020032 OTTMUSG00000022766
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000020220 protein_coding No protein product (NMD) view

Predicted translation:

MEGAAVSAAGDGPAVETGLPGSPLEAVAGATAAPVEPRKPHGVKRHHHKHNLKHRYELQETLGKGTYGKVKRATERFSGR
VCLRTKIRL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000574455 Prot_kinase_cat_dom (25/251 aa) | Ser-Thr/Tyr_kinase_cat_dom (24/247 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000574454 Prot_kinase_cat_dom (41/251 aa) | Ser-Thr/Tyr_kinase_cat_dom (41/247 aa) | LipoPS_kinase (37/114 aa) CDS Deleted
ENSMUSE00000574453 Prot_kinase_cat_dom (51/251 aa) | Ser-Thr/Tyr_kinase_cat_dom (51/247 aa) | LipoPS_kinase (51/114 aa) CDS CDS + stop codon + UTR
ENSMUSE00000574452 Prot_kinase_cat_dom (22/251 aa) | Ser-Thr/Tyr_kinase_cat_dom (22/247 aa) | LipoPS_kinase (22/114 aa) CDS
ENSMUSE00000438104 Prot_kinase_cat_dom (40/251 aa) | Ser-Thr/Tyr_kinase_cat_dom (40/247 aa) | LipoPS_kinase (5/114 aa) CDS
ENSMUSE00000100003 Prot_kinase_cat_dom (45/251 aa) | Ser-Thr/Tyr_kinase_cat_dom (45/247 aa) CDS
ENSMUSE00000506302 Prot_kinase_cat_dom (29/251 aa) | Ser-Thr/Tyr_kinase_cat_dom (26/247 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000129355 protein_coding Target region does not overlap transcript
show/hide more transcripts

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available