IKMC Project Report - EUCOMM (ID: 78864)

Klhl25 MGI:2668031 ENSMUSG00000055652
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000092073 protein_coding Novel (out of frame) product view

Predicted translation:

MKFSSVTCALVSKAGSGSCGEKRPEARAGTSNQRHLCRALRLIVTQAWQWLPGKSRGPLAQQHSL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000634123 UTR UTR
ENSMUSE00000447883 Kelch_1 (46/46 aa) | Kelch_1 (55/55 aa) | Kelch_1 (43/43 aa) | Kelch_1 (42/42 aa) | Kelch_1 (41/41 aa) | BACK (102/102 aa) | Kelch_2 (54/54 aa) | Kelch_2 (44/44 aa) | BTB_POZ (108/108 aa) Deleted
ENSMUSE00000573328 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000171155 protein_coding Novel (out of frame) product view

Predicted translation:

MKFSSVTCALVSKAGSGSCGEKRPEARAGTSNQRHLCRALRLIVTQAWQWLPGKSRGPLAQQHSL*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000997872 UTR UTR
ENSMUSE00000447883 Kelch_1 (46/46 aa) | Kelch_1 (55/55 aa) | Kelch_1 (43/43 aa) | Kelch_1 (42/42 aa) | Kelch_1 (41/41 aa) | BACK (102/102 aa) | Kelch_2 (54/54 aa) | Kelch_2 (44/44 aa) | BTB_POZ (108/108 aa) Deleted
ENSMUSE00000573328 UTR UTR
Legend: in frame deleted frameshifted
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available