IKMC Project Report - KOMP-CSD (ID: 79312)
Akr1c21 MGI:1924587 ENSMUSG00000021207 OTTMUSG00000027696
Mice no data available
Mutagenesis Predictions
This gene has 2 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.
| Ensembl transcript id | Ensembl Biotype | Floxed transcript description | Details | |||||||||||||||||||||||||||||||||||||||||||||||
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| ENSMUST00000021628 | protein_coding | Residual N-terminal, residual C-terminal product | view | |||||||||||||||||||||||||||||||||||||||||||||||
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Predicted translation: MNSKCHCVILNDGNFIPVLGFGTALPLEVECHPYLNQMKLLDFCKSKDIVLVAYGVLGTQRYGGWVDQNSPVLLDEPVLG SMAKKYNRTPALIALRYQLQRGIVVLNTSLKEERIKENMQVFEFQLSSEDMKVLDGLNRNMRYIPAAIFKGHPNWPFLDE Y*
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| ENSMUST00000137279 | retained_intron | No analysis available: incomplete transcript | ||||||||||||||||||||||||||||||||||||||||||||||||
ES Cell Clones With Knockout First Mutation (Reporter Tagged Insertion With Conditional Potential)
| Genome Browsers: | Ensembl (mouse) - UCSC (mouse) |
|---|---|
| Tools: | Southern Blot Tool - LRPCR Genotyping Primers |
| ES Cell Clone | Targeting Vector | Allele | Allele Type | Parental ES Cell Line | Genbank File | Mouse | QC Data | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | EPD0604_2_G11 | HTGRS6005_A_D02 | Akr1c21tm1a(KOMP)Wtsi | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | JM8A3.N1 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ES Cell Clones Without Conditional Potential
Note: Mutations of type "Without Conditional Potential" are correctly targeted clones that have lost the 3' LoxP site. These mutations cannot be converted into conditional alleles.
| Genome Browsers: | Ensembl (mouse) - UCSC (mouse) |
|---|---|
| Tools: | Southern Blot Tool - LRPCR Genotyping Primers |
| ES Cell Clone | Targeting Vector | Allele | Allele Type | Parental ES Cell Line | Genbank File | Mouse | QC Data | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| order | EPD0604_2_E10 | HTGRS6005_A_D02 | Akr1c21tm1e(KOMP)Wtsi | Targeted Non-Conditional (Promotor Driven Cassette) | JM8A3.N1 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| order | EPD0604_2_A09 | HTGRS6005_A_D02 | Akr1c21tm1e(KOMP)Wtsi | Targeted Non-Conditional (Promotor Driven Cassette) | JM8A3.N1 | view | no | view ( about ) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Targeting Vectors
| Genome Browsers: | Ensembl (mouse) - UCSC (mouse) |
|---|---|
| Tools: | LRPCR Genotyping Primers |
| Design ID | Vector Type | Targeting Vector | Cassette | Backbone | Genbank File | |
|---|---|---|---|---|---|---|
| order | 103078 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | HTGR06005_A_1_D02 | L1L2_Bact_P | R3R4_pBR_DTA+_Bsd_amp | view |
| order | 103078 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | HTGR06005_A_2_D02 | L1L2_Bact_P | R3R4_pBR_DTA+_Bsd_amp | view |
| order | 103078 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | HTGRS6005_A_D02 | L1L2_Bact_P | R3R4_pBR_DTA+_Bsd_amp | view |
| order | 103078 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | HTGR06005_A_7_D02 | L1L2_Bact_P | R3R4_pBR_DTA+_Bsd_amp | view |
| order | 103078 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | HTGR06005_A_6_D02 | L1L2_Bact_P | R3R4_pBR_DTA+_Bsd_amp | view |
| order | 103078 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | HTGR06005_A_5_D02 | L1L2_Bact_P | R3R4_pBR_DTA+_Bsd_amp | view |
| order | 103078 | Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) | HTGR06005_A_3_D02 | L1L2_Bact_P | R3R4_pBR_DTA+_Bsd_amp | view |
Intermediate Vectors
| Design ID | Vector Type | Intermediate Vector |
|---|---|---|
| 103078 | Knockout First, Reporter-tagged insertion with conditional potential | PCS6005_A_D02 |