IKMC Project Report - KOMP-CSD (ID: 79455)

Wap MGI:98943 ENSMUSG00000000381 OTTMUSG00000005118
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Targeting Confirmed

Mice no data available

Mutagenesis Predictions

This gene has 3 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000102910 protein_coding Residual N-terminal, residual C-terminal product view

Predicted translation: 

MRCLISLVLGLLALEVALAQNLEEQVFNSVITLQ*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000799697 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000104649 CDS Deleted
ENSMUSE00000991308 Whey_acidic_protein_4-diS_core (49/49 aa) CDS Deleted
ENSMUSE00001061683 CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000141868 processed_transcript No analysis available: incomplete transcript
ENSMUST00000127341 processed_transcript No analysis available: incomplete transcript
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ES Cell Clones With Conditional Potential

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool - LRPCR Genotyping Primers
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order EPD0707_5_B04 HTGRS06009_A_D06 Waptm1a(KOMP)Wtsi Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order EPD0707_5_G01 HTGRS06009_A_D06 Waptm1a(KOMP)Wtsi Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order EPD0707_5_F04 HTGRS06009_A_D06 Waptm1a(KOMP)Wtsi Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order EPD0707_5_C04 HTGRS06009_A_D06 Waptm1a(KOMP)Wtsi Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGRS06009_A_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR06009_A_1_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR06009_A_8_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR06009_A_2_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR06009_A_7_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR06009_A_4_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR06009_A_6_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR06009_A_5_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 119129 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) HTGR06009_A_3_D06 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
119129 Knockout-First - Reporter Tagged Insertion PCS6009_A_D06