International Knockout Mouse Consortium - MartSearch

• Home
• About IKMC
• MartSearch
• Download
• Nominate Gene
• FAQ
• Order Products
• Contact IKMC

• MartSearch
• Home
• Browse
• Help
• About

IKMC Project Report - KOMP-CSD (ID: 79984)

Serpinb5 MGI:109579 ENSMUSG00000067006 OTTMUSG00000020990

Program: KOMP-CSD
Pipeline Progress: ES Cells - Targeting Confirmed

	Pre-pipeline	Designs	Vectors	ES Cells	Mice
				ES Cells - Targeting Confirmed

Mice no data available

Mutagenesis Predictions no data available

ES Cell Clones With Conditional Potential

Genome Browsers:	Ensembl (mouse) - UCSC (mouse)
Tools:	Southern Blot Tool - LRPCR Genotyping Primers

	ES Cell Clone	Targeting Vector	Allele	Allele Type	Parental ES Cell Line	Genbank File	Mouse	QC Data
order	EPD0706_4_B11	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen pass Loss of WT Allele (LOA) - Vector Integrity pass Distribution Centre Karyotype - Copy Number pass Cells Thawed Correctly - 5' LR-PCR pass 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_B10	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen pass Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_A10	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen pass Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_C09	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen not confirmed LoxP Screen pass 3' Screen pass Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_H10	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen pass Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_H12	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen pass Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_G12	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen pass Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_A09	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen pass Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_G10	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen not confirmed Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_H09	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen not confirmed Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_F12	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen not confirmed Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_F11	HTGRS6015_A_D08	Serpinb5tm1a(KOMP)Wtsi	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen pass 3' Screen not confirmed Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -

show/hide more ES cells

ES Cell Clones Without Conditional Potential

Note: Mutations of type "Without Conditional Potential" are correctly targeted clones that have lost the 3' LoxP site. These mutations cannot be converted into conditional alleles.

Genome Browsers:	Ensembl (mouse) - UCSC (mouse)
Tools:	Southern Blot Tool - LRPCR Genotyping Primers

	ES Cell Clone	Targeting Vector	Allele	Allele Type	Parental ES Cell Line	Genbank File	Mouse	QC Data
order	EPD0706_4_H11	HTGRS6015_A_D08	Serpinb5tm1e(KOMP)Wtsi	Targeted Non-Conditional (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -
order	EPD0706_4_G11	HTGRS6015_A_D08	Serpinb5tm1e(KOMP)Wtsi	Targeted Non-Conditional (Promotor Driven Cassette)	JM8A3.N1	view	no	view    ( about )
Production Centre 5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed Loss of WT Allele (LOA) - Vector Integrity - Distribution Centre Karyotype - Copy Number - Cells Thawed Correctly - 5' LR-PCR - 5' SR-PCR - 3' SR-PCR - LOA - LOXP - LACZ - Chromosome 1 - Chromosome 8a - Chromosome 8b - Chromosome 11a - Chromosome 11b - Chromosome Y - User/Mouse Clinic Southern Blot - Map Test - Karyotype - TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) - Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity - Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation - 3' LR-PCR -

show/hide more ES cells

Targeting Vectors

Genome Browsers:	Ensembl (mouse) - UCSC (mouse)
Tools:	LRPCR Genotyping Primers

	Design ID	Vector Type	Targeting Vector	Cassette	Backbone	Genbank File
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGR06015_A_2_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGR06015_A_6_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGRS6015_A_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGR06015_A_1_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGR06015_A_7_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGR06015_A_8_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGR06015_A_4_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGR06015_A_3_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
order	87143	Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)	HTGR06015_A_5_D08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view

show/hide more targeting vectors

Intermediate Vectors

Design ID	Vector Type	Intermediate Vector
87143	Knockout-First - Reporter Tagged Insertion	PCS6015_A_D08

Warranty Disclaimer and Copyright Notice
This site is maintained by the I-DCC and the KOMP-DCC
Supported by the European Union (Project number: 223592)
and the National Institutes of Health (Grant number: NIH HG004074)

1.1.44