IKMC Project Report - KOMP-CSD (ID: 80175)

Atp5k MGI:106636 ENSMUSG00000050856 OTTMUSG00000028932
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 3 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). This mutation is of type 'Deletion' (more information on IKMC alleles can be found here). The table below shows the predicted structure of the gene transcripts. Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000049628 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MVPPVQVSPLIK

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000715057 ATPase_F0-cplx_esu_mt (10/48 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000365706 ATPase_F0-cplx_esu_mt (19/48 aa) CDS Deleted
ENSMUSE00001002286 ATPase_F0-cplx_esu_mt (20/48 aa) CDS Deleted
ENSMUSE00000721000 CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000118632 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MVPPVQVSPLIK

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000715057 ATPase_F0-cplx_esu_mt (10/31 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000365706 ATPase_F0-cplx_esu_mt (19/31 aa) CDS Deleted
ENSMUSE00000721000 ATPase_F0-cplx_esu_mt (3/31 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000123184 retained_intron No analysis available: incomplete transcript
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ES Cell Clones Without Conditional Potential (Deletions)

Note: Mutations of type "Deletion" are correctly targeted clones that have had the target exon removed. These mutations cannot be converted into conditional alleles.

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool - LRPCR Genotyping Primers
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order EPD0710_2_B12 HTGRS6003_A_C08 Atp5ktm1(EUCOMM)Wtsi Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order EPD0710_2_A11 HTGRS6003_A_C08 Atp5ktm1(EUCOMM)Wtsi Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order EPD0710_2_D09 HTGRS6003_A_C08 Atp5ktm1(EUCOMM)Wtsi Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order EPD0710_2_H12 HTGRS6003_A_C08 Atp5ktm1(EUCOMM)Wtsi Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order EPD0710_2_F10 HTGRS6003_A_C08 Atp5ktm1(EUCOMM)Wtsi Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order EPD0710_2_D11 HTGRS6003_A_C08 Atp5ktm1(EUCOMM)Wtsi Deletion (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 213889 Deletion (Promotor Driven Cassette) HTGRS6003_A_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 213889 Deletion (Promotor Driven Cassette) HTGR06003_A_2_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 213889 Deletion (Promotor Driven Cassette) HTGR06003_A_7_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 213889 Deletion (Promotor Driven Cassette) HTGR06003_A_1_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 213889 Deletion (Promotor Driven Cassette) HTGR06003_A_8_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 213889 Deletion (Promotor Driven Cassette) HTGR06003_A_3_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 213889 Deletion (Promotor Driven Cassette) HTGR06003_A_4_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 213889 Deletion (Promotor Driven Cassette) HTGR06003_A_6_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 213889 Deletion (Promotor Driven Cassette) HTGR06003_A_5_C08 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
213889 Deletion PCS6003_A_C08