IKMC Project Report - EUCOMM (ID: 81228)

Wasf2 MGI:1098641 ENSMUSG00000028868 OTTMUSG00000010477
Pre-pipeline Designs Vectors ES Cells Mice
Vector Construction in Progress

Mice no data available

Mutagenesis Predictions

This gene has 6 wild type transcripts, of which 3 are protein-coding. Following removal of the floxed region, 3 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000084241 protein_coding No protein product (NMD) view

Predicted translation:

MPLVTRNIEPRHLCRQTLPSDTSELECRTNITLANVIRQLGSLSKYAEDIFGEICTQASAFASRVNSLAERVDRVQVKVT
QLDPKEEEVSLQGINTRKAFRSSTTQDQKLFDRNSLPVPVLETYNSCDAPPPLNNLSPYRKKRKIIQIEGM*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000821621 UTR UTR
ENSMUSE00001030790 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000182945 CDS CDS
ENSMUSE00000182948 CDS CDS
ENSMUSE00000182960 CDS Deleted
ENSMUSE00000182942 CDS CDS + stop codon + UTR
ENSMUSE00000182963 CDS
ENSMUSE00000331657 WH2_dom (11/18 aa) CDS
ENSMUSE00000524637 WH2_dom (8/18 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000105912 protein_coding No protein product (NMD) view

Predicted translation:

MPLVTRNIEPRHLCRQTLPSDTSELECRTNITLANVIRQLGSLSKYAEDIFGEICTQASAFASRVNSLAERVDRVQVKVT
QLDPKEEEVSLQGINTRKAFRSSTTQDQKLFDRNSLPVPVLETYNSCDAPPPLNNLSPYRKKRKIIQIEGM*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000631012 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000182945 CDS CDS
ENSMUSE00000182948 CDS CDS
ENSMUSE00000182960 CDS Deleted
ENSMUSE00000182942 CDS CDS + stop codon + UTR
ENSMUSE00000182963 CDS
ENSMUSE00000331657 WH2_dom (11/18 aa) CDS
ENSMUSE00000667963 WH2_dom (8/18 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000138831 protein_coding Target region does not overlap transcript
ENSMUST00000136637 processed_transcript No analysis available: incomplete transcript
ENSMUST00000136132 processed_transcript Target region does not overlap transcript
ENSMUST00000123578 processed_transcript Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available