IKMC Project Report - KOMP-CSD (ID: 82200)

1700007K09Rik MGI:1916568 ENSMUSG00000030858
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000033146 protein_coding Design floxes first exon in transcript ENSMUST00000033146

ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 96792 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00210_Z_5_G07 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 96792 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00210_Z_4_G07 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 96792 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00210_Z_2_G07 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 96792 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00210_Z_1_G07 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 96792 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PRPGS00210_A_G07 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 96792 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00210_Z_7_G07 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 96792 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00210_Z_6_G07 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
96792 Knockout-First - Reporter Tagged Insertion PCS00210_A_G07