IKMC Project Report - EUCOMM (ID: 83016)

1700007B14Rik MGI:1919081 ENSMUSG00000031620 OTTMUSG00000031095
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Targeting Confirmed

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000034033 protein_coding No protein product (NMD) view

Predicted translation: 

MAPAEAIPEGVGPVLETTQQDFLQEAKSLIAQHHKRLNENKAQGTSIDVFKNKHQKPKTVKFIPFEVKKSEILNVVQEHR
LVLKSISYPRDTSKCTALIEASQQISFKEPHVSNIKEKCHPVDLLKEEKVKLGKIMNDIESVSKQMEMEKEMHLRKSRML
EPFHSHMSLPLHNLASSLGLLKELADRTLYDWRSAALIRSSYLPHGSAQSLSESSVTFKNYYKRPIRKKDLQPIKKELKT
EKRFRIMGQI*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000682106 UTR UTR
ENSMUSE00000682105 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000380983 CDS CDS
ENSMUSE00000250852 CDS CDS
ENSMUSE00000391111 CDS CDS
ENSMUSE00000211189 CDS CDS
ENSMUSE00000211193 CDS CDS
ENSMUSE00000211192 CDS CDS
ENSMUSE00000211190 CDS Deleted
ENSMUSE00000211187 CDS CDS + stop codon + UTR
ENSMUSE00000211191 CDS
ENSMUSE00000250815 CDS
ENSMUSE00000357281 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000121983 protein_coding No protein product (NMD) view

Predicted translation: 

MAPAEAIPEGVGPVLETTQQDFLQEAKSLIAQHHKRLNENKAQGTSIDVFKNKHQKPKTVKFIPFEVKKSEILNVVQEHR
LVLKSISYPRDTSKCTALIEASQQISFKEPHVSNIKEKCHPVDLLKEEKVKLGKIMNDIESVSKQMEMEKEMHLRKSRML
EPFHSHMSLPLHNLASSLGLLKELADRTLYDWRSAALIRSSYLPHGSAQSLSESSVTFKNYYKRPIRKKDLQPIKKELKT
EKRFRIMGQI*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000713591 UTR UTR
ENSMUSE00000715260 UTR UTR
ENSMUSE00000710302 UTR UTR
ENSMUSE00000682105 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000380983 CDS CDS
ENSMUSE00000250852 CDS CDS
ENSMUSE00000391111 CDS CDS
ENSMUSE00000211189 CDS CDS
ENSMUSE00000211193 CDS CDS
ENSMUSE00000211192 CDS CDS
ENSMUSE00000211190 CDS Deleted
ENSMUSE00000211187 CDS CDS + stop codon + UTR
ENSMUSE00000211191 CDS
ENSMUSE00000250815 CDS
ENSMUSE00000711509 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
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ES Cell Clones With Knockout First Mutation (Reporter Tagged Insertion With Conditional Potential)

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool - LRPCR Genotyping Primers
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order HEPD0692_1_C06 PG00217_Z_6_B06 1700007B14Riktm1a(EUCOMM)Hmgu Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

ES Cell Clones Without Conditional Potential

Note: Mutations of type "Without Conditional Potential" are correctly targeted clones that have lost the 3' LoxP site. These mutations cannot be converted into conditional alleles.

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool - LRPCR Genotyping Primers
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order HEPD0692_1_B06 PG00217_Z_6_B06 1700007B14Riktm1e(EUCOMM)Hmgu Targeted Non-Conditional (Promotor Driven Cassette) JM8A3.N1 view no view    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 270592 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) PG00217_Z_6_E12 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
order 270592 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) PG00217_Z_6_A01 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
order 270592 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) PG00217_Z_6_B06 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
270592 Knockout First, Reporter-tagged insertion with conditional potential PCS00217_A_F01