IKMC Project Report - EUCOMM (ID: 88096)

Taar3 MGI:3527427 ENSMUSG00000069708 OTTMUSG00000019213
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000045152 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MDLIYIPEDLSSCPKFGNKSCPPTNRSFRVRMIMYLFMTGAMVITIFGNLVIIISISHFKQLHSPTNFLILSMATTDFLL
GFVIMPYSMVRSVE

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000286213 7TM_GPCR_Rhodpsn (46/261 aa) | 7TM_GPCR_olfarory/Srsx (52/248 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000286213 7TM_GPCR_Rhodpsn (216/261 aa) | 7TM_GPCR_olfarory/Srsx (197/248 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_A01 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_A06 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_E08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_D08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_B01 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_A04 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_C02 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_A09 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_C10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_E10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_G06 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_G08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_D07 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_F02 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_C08 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_C12 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_F03 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_H07 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_F10 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_G12 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_E07 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_H01 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
order 377986 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00254_Z_1_D05 Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_spec view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
377986 Knockout-First - Reporter Tagged Insertion PC00254_A_D03