IKMC Project Report - KOMP-CSD (ID: 89523)

Ago3 MGI:2446634 ENSMUSG00000028842 OTTMUSG00000009349
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 6 wild type transcripts, of which 3 are protein-coding. Following removal of the floxed region, 3 transcripts are predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000069097 protein_coding No protein product (NMD) view

Predicted translation:

MEIGSAGPIGAQPLFIVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNR*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000788193 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001091569 CDS CDS
ENSMUSE00000631279 CDS Deleted
ENSMUSE00000631278 CDS CDS + stop codon + UTR
ENSMUSE00000975218 DUF1785 (44/53 aa) CDS
ENSMUSE00001003440 PAZ (28/134 aa) | DUF1785 (10/53 aa) CDS
ENSMUSE00000631275 PAZ (30/134 aa) CDS
ENSMUSE00000631274 PAZ (50/134 aa) CDS
ENSMUSE00001006869 PAZ (28/134 aa) CDS
ENSMUSE00000234863 CDS
ENSMUSE00000631272 CDS
ENSMUSE00000384103 Piwi (12/300 aa) CDS
ENSMUSE00000977673 Piwi (54/300 aa) CDS
ENSMUSE00000631271 Piwi (31/300 aa) CDS
ENSMUSE00000631270 Piwi (65/300 aa) CDS
ENSMUSE00000631269 Piwi (45/300 aa) CDS
ENSMUSE00000631268 Piwi (34/300 aa) CDS
ENSMUSE00001048424 Piwi (61/300 aa) CDS
ENSMUSE00000807422 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000132123 protein_coding Target region does not overlap transcript
ENSMUST00000127831 protein_coding Target region does not overlap transcript
ENSMUST00000155645 processed_transcript Target region does not overlap transcript
ENSMUST00000151179 processed_transcript Target region does not overlap transcript
ENSMUST00000123233 processed_transcript No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available