IKMC Project Report - KOMP-CSD (ID: 90023)

Gpx3 MGI:105102 ENSMUSG00000018339 OTTMUSG00000006337
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 3 wild type transcripts, of which 3 are protein-coding. Following removal of the floxed region, 3 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000082430 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MARILRASCLLSLLLAGFVPPGRGQEKSK

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000739745 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000660038 Glutathione_peroxidase (41/114 aa) CDS Deleted
ENSMUSE00000660039 Glutathione_peroxidase (40/114 aa) CDS Deleted
ENSMUSE00000660040 Glutathione_peroxidase (34/114 aa) CDS Deleted
ENSMUSE00000660041 Glutathione_peroxidase (1/114 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000149324 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MARILRASCLLSLLLAGFVPPGRGQEKSK

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000660037 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000660038 Glutathione_peroxidase (41/41 aa) CDS Deleted
ENSMUSE00000832642 Glutathione_peroxidase (1/41 aa) | Glutathione_peroxidase (10/83 aa) CDS Deleted
ENSMUSE00000660039 Glutathione_peroxidase (40/83 aa) CDS Deleted
ENSMUSE00000660040 Glutathione_peroxidase (34/83 aa) CDS Deleted
ENSMUSE00000843533 Glutathione_peroxidase (1/83 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000125094 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MARILRASCLLSLLLAGFVPPGRGQEKSK

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000760829 UTR UTR
ENSMUSE00000797406 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000660038 Glutathione_peroxidase (41/114 aa) CDS Deleted
ENSMUSE00000660039 Glutathione_peroxidase (40/114 aa) CDS Deleted
ENSMUSE00000660040 Glutathione_peroxidase (34/114 aa) CDS Deleted
ENSMUSE00000785058 Glutathione_peroxidase (1/114 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
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ES Cell Clones no data available

Targeting Vectors

Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00233_Z_4_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00233_Z_8_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00233_Z_7_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00233_Z_1_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00233_Z_3_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00233_Z_6_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PRPGS00233_A_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00233_Z_2_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
order 357974 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00233_Z_5_G09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
357974 Knockout-First - Reporter Tagged Insertion PCS00233_A_G09