IKMC Project Report - EUCOMM (ID: 92082)

1600012P17Rik MGI:1919275 ENSMUSG00000047661 OTTMUSG00000030139
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout First, Reporter-tagged insertion with conditional potential'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000062159 protein_coding No protein product
ENSMUST00000162474 protein_coding No protein product
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ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 418203 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_5_F08 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
order 418203 Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) ETPG00284_U_5_E02 L1L2_GT0_LF2A_LacZ_BetactP_neo L3L4_pD223_DTA_T_spec view
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Intermediate Vectors no data available