IKMC Project Report - EUCOMM (ID: 92630)

Wnt8a MGI:107924 ENSMUSG00000012282
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - No QC Positives

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000012426 protein_coding Residual N-terminal, novel C-terminal product view

Predicted translation:

MGHLLMLWVAAGMCYPALGASAWSVNNFLITRPKAYLTYTASVALGAQIGIEECKFQFAWERWNCPEHAFQFSTHNRLRA
GSEGLHEKDLQVSWHLRKLQHPDVLAAAG*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000395296 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000572801 CDS CDS
ENSMUSE00000141473 Wnt (45/301 aa) CDS CDS
ENSMUSE00000141469 Wnt (43/301 aa) CDS Deleted
ENSMUSE00000141475 Wnt (48/301 aa) CDS Deleted
ENSMUSE00000331919 Wnt (167/301 aa) CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors

Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 366816 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00239_Z_8_F06 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
order 366816 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00239_Z_8_F01 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
366816 Knockout-First - Reporter Tagged Insertion PCS00239_A_H11