IKMC Project Report - EUCOMM (ID: 92657)

1600029D21Rik MGI:1923759 ENSMUSG00000032068
Pre-pipeline Designs Vectors ES Cells Mice
Vector Complete

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type 'Knockout-First - Reporter Tagged Insertion'. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000114474 protein_coding Residual N-terminal, novel C-terminal product view

Predicted translation: 

MLSLRSLLPHLGLFLCLALHLSPSLSASDNGSCVVLDNIYTSDILEISTMANVSGGDVTYTGSSSSSIAQPQSHP*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000518724 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000216248 CDS Deleted
ENSMUSE00000216252 CDS CDS + stop codon + UTR
ENSMUSE00000699475 CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_H02 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_E11 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_3_H04 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_3_G03 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_G06 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_3_G10 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_H10 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_B02 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_A06 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_C03 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_B10 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_G12 L1L2_Bact_P L3L4_pD223_DTA_spec view
order 281968 Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette) PG00222_Y_1_F11 L1L2_Bact_P L3L4_pD223_DTA_spec view
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Intermediate Vectors

Design ID Vector Type Intermediate Vector
281968 Knockout-First - Reporter Tagged Insertion PCS00222_A_A11