IKMC Project Report - EUCOMM (ID: 93879)

Acadm MGI:87867 ENSMUSG00000062908 OTTMUSG00000031346
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 6 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000072697 protein_coding No protein product (NMD) view

Predicted translation:

MAAAFRRGCRVLRSVSHFECRTQHSKAAHKQEPGLGFSFELTEQQKEFQATARKFAREEIIPVAPEYDKSGEYPFPLIKR
AWELGLINAHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGVFLVGTF*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000790909 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000499736 CDS CDS
ENSMUSE00000500621 Acyl-CoA_DH_N (30/110 aa) CDS CDS
ENSMUSE00000177205 Acyl-CoA_DH_N (24/110 aa) CDS CDS
ENSMUSE00001087043 Acyl-CoA_DH_N (34/110 aa) CDS CDS
ENSMUSE00000989376 Acyl-CoA_DH_N (23/110 aa) CDS Deleted
ENSMUSE00000177200 Acyl-CoA_Oxase/DH_cen-dom (43/51 aa) CDS Deleted
ENSMUSE00000498305 Acyl-CoA_Oxase/DH_cen-dom (9/51 aa) CDS CDS + stop codon + UTR
ENSMUSE00000491379 Acyl-CoA_Oxase/DH_1 (16/148 aa) CDS
ENSMUSE00000493257 Acyl-CoA_Oxase/DH_1 (32/148 aa) | Acyl-CoA_DH_2_C (31/120 aa) CDS
ENSMUSE00000177201 Acyl-CoA_Oxase/DH_1 (83/148 aa) | Acyl-CoA_DH_2_C (83/120 aa) CDS
ENSMUSE00000383242 Acyl-CoA_Oxase/DH_1 (17/148 aa) | Acyl-CoA_DH_2_C (6/120 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000150070 protein_coding No analysis available: incomplete transcript
ENSMUST00000156310 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000137161 retained_intron Target region does not overlap transcript
ENSMUST00000130713 retained_intron Target region does not overlap transcript
ENSMUST00000135724 retained_intron Target region does not overlap transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available