IKMC Project Report - KOMP-CSD (ID: 94295)

Aif1 MGI:1343098 ENSMUSG00000024397 OTTMUSG00000037184
Pre-pipeline Designs Vectors ES Cells Mice
Vector Construction in Progress

Mice no data available

Mutagenesis Predictions

This gene has 7 wild type transcripts, of which 4 are protein-coding. Following removal of the floxed region, 4 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000025257 protein_coding Novel C-terminal product view

Predicted translation:

MRRKTKNTRGQLVPQPRKLSPSCPDWRWMSHGGAE*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00001003881 UTR UTR
ENSMUSE00000141961 UTR + start codon + CDS
ENSMUSE00000968894 CDS Deleted
ENSMUSE00000981172 CDS Deleted
ENSMUSE00000993844 CDS Deleted
ENSMUSE00000985756 CDS Deleted
ENSMUSE00000934105 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000173324 protein_coding Novel C-terminal product view

Predicted translation:

MRRKTKNTRGQLVPQPRKLSPSCPDWRWMSHGGAE*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000942006 UTR UTR
ENSMUSE00000927616 UTR UTR
ENSMUSE00000141961 UTR + start codon + CDS
ENSMUSE00000968894 CDS Deleted
ENSMUSE00000981172 CDS Deleted
ENSMUSE00000993844 CDS Deleted
ENSMUSE00000985756 CDS Deleted
ENSMUSE00000141964 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000172693 protein_coding Novel C-terminal product view

Predicted translation:

MRRKTKNTRGQLVPQPRKLSPSCPDWRWMSHGGAE*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000938584 UTR + start codon + CDS
ENSMUSE00000968894 CDS Deleted
ENSMUSE00000981172 CDS Deleted
ENSMUSE00000993844 CDS Deleted
ENSMUSE00000985756 CDS Deleted
ENSMUSE00001071635 CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000173106 protein_coding No analysis available: incomplete transcript
ENSMUST00000174044 retained_intron Target region does not overlap transcript
ENSMUST00000172679 processed_transcript No analysis available: incomplete transcript
ENSMUST00000173281 retained_intron No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available