IKMC Project Report - EUCOMM (ID: 98965)

Wasf3 MGI:2658986 ENSMUSG00000029636 OTTMUSG00000025851
Pre-pipeline Designs Vectors ES Cells Mice
Vector Construction in Progress

Mice no data available

Mutagenesis Predictions

This gene has 2 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000016143 protein_coding No protein product (NMD) view

Predicted translation:

MPLVKRNIEPRHLCRGALPEGVTSELECVTNSTLAAIIRQLSSLSKHAEDIFGELFNEANNFYIRANSLQDRIDRLAVKV
TQLDSTVEEEMTRRMG*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000494684 UTR UTR
ENSMUSE00000493556 UTR UTR
ENSMUSE00000496411 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000494909 CDS CDS
ENSMUSE00000497754 CDS Deleted
ENSMUSE00000496725 CDS CDS + stop codon + UTR
ENSMUSE00000499597 CDS
ENSMUSE00000263795 CDS
ENSMUSE00000263789 WH2_dom (11/18 aa) CDS
ENSMUSE00000647761 WH2_dom (8/18 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000110600 protein_coding Target region does not overlap transcript
show/hide more transcripts

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available