IKMC Project: VG10115 - International Knockout Mouse Consortium

IKMC Project Report - KOMP-Regeneron (ID: VG10115)

Lrfn4 MGI:2385612 ENSMUSG00000045045 OTTMUSG00000014906

	Pre-pipeline	Designs	Vectors	ES Cells	Mice
					Germline Transmission Achieved

Mice

Microinjection Status

Allele

Allele Type

ES Cell Clone

Genetic Background

QC Data

MI/Distribution Centre

contact distributor

Genotype confirmed

Lrfn4^{tm1(KOMP)Vlcg}

Deletion (Promotor Driven Cassette)

10115A-A9

C57BL/6N

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( about )

UCD

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	na	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	na	LoxP Confirmation	na	3' LR-PCR
Genotyping Comment	-

Mutagenesis Predictions no data available

ES Cell Clones Without Conditional Potential (Deletions)

Note: Mutations of type "Deletion" are correctly targeted clones that have had the target exon removed. These mutations cannot be converted into conditional alleles.

Genome Browsers:	Ensembl (mouse) - UCSC (mouse)
Tools:	Southern Blot Tool

	ES Cell Clone	Targeting Vector	Allele	Allele Type	Parental ES Cell Line	Genbank File	Mouse	QC Data
order	10115A-A9	VG10115	Lrfn4^{tm1(KOMP)Vlcg}	Deletion (Promotor Driven Cassette)	VGB6	view	yes	no data reported ( about )
order	10115A-A11	VG10115	Lrfn4^{tm1(KOMP)Vlcg}	Deletion (Promotor Driven Cassette)	VGB6	view	no	no data reported ( about )
order	10115A-A2	VG10115	Lrfn4^{tm1(KOMP)Vlcg}	Deletion (Promotor Driven Cassette)	VGB6	view	no	no data reported ( about )
order	10115A-B2	VG10115	Lrfn4^{tm1(KOMP)Vlcg}	Deletion (Promotor Driven Cassette)	VGB6	view	no	no data reported ( about )
order	10115A-B8	VG10115	Lrfn4^{tm1(KOMP)Vlcg}	Deletion (Promotor Driven Cassette)	VGB6	view	no	no data reported ( about )
order	10115A-D7	VG10115	Lrfn4^{tm1(KOMP)Vlcg}	Deletion (Promotor Driven Cassette)	VGB6	view	no	no data reported ( about )

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Targeting Vectors

Genome Browsers:	Ensembl (mouse) - UCSC (mouse)

	Design ID	Vector Type	Targeting Vector	Cassette	Backbone	Genbank File
order	-	Deletion (Promotor Driven Cassette)	VG10115	ZEN-UB1.GB		view