IKMC Project Report - KOMP-Regeneron (ID: VG10337)

Slc7a13 MGI:1921337 ENSMUSG00000041052 OTTMUSG00000006379
Pre-pipeline Designs Vectors ES Cells Mice
Germline Transmission Achieved

Mice

Microinjection Status Allele Allele Type ES Cell Clone Genetic Background QC Data MI/Distribution Centre
currently unavailable Genotype confirmed Slc7a13tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) 10337A-D1 C57BL/6N view
( about ) 		UCD
Southern Blot na TV Backbone Assay na 5' LR-PCR na
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) na
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR na LoxP Confirmation na 3' LR-PCR
Genotyping Comment -
currently unavailable Genotype confirmed Slc7a13tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) 10337A-H4 C57BL/6N view
( about ) 		UCD
Southern Blot na TV Backbone Assay na 5' LR-PCR na
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) na
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR na LoxP Confirmation na 3' LR-PCR
Genotyping Comment -

Mutagenesis Predictions no data available

ES Cell Clones Without Conditional Potential (Deletions)

Note: Mutations of type "Deletion" are correctly targeted clones that have had the target exon removed. These mutations cannot be converted into conditional alleles.

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order 10337A-D1 VG10337 Slc7a13tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view yes no data reported    ( about )
order 10337A-H4 VG10337 Slc7a13tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view yes no data reported    ( about )
order 10337A-H6 VG10337 Slc7a13tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view no no data reported    ( about )
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order - Deletion (Promotor Driven Cassette) VG10337 ZEN-UB1.GB view

Intermediate Vectors no data available