IKMC Project Report - KOMP-Regeneron (ID: VG11658)

Cyp8b1 MGI:1338044 ENSMUSG00000050445 OTTMUSG00000022966
Pre-pipeline Designs Vectors ES Cells Mice
Germline Transmission Achieved

Mice

Microinjection Status Allele Allele Type ES Cell Clone Genetic Background QC Data MI/Distribution Centre
currently unavailable Genotype confirmed Cyp8b1tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) 11658A-D5 C57BL/6NTac;C57BL/6NTac;C57BL/6N view
( about ) 		UCD/
Southern Blot na TV Backbone Assay na 5' LR-PCR na
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) pass
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR na LoxP Confirmation na 3' LR-PCR
Genotyping Comment -

Mutagenesis Predictions no data available

ES Cell Clones Without Conditional Potential (Deletions)

Note: Mutations of type "Deletion" are correctly targeted clones that have had the target exon removed. These mutations cannot be converted into conditional alleles.

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order 11658A-D5 VG11658 Cyp8b1tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view yes no data reported    ( about )
order 11658A-F7 VG11658 Cyp8b1tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view no no data reported    ( about )
order 11658A-G9 VG11658 Cyp8b1tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view no no data reported    ( about )
order 11658A-H3 VG11658 Cyp8b1tm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view no no data reported    ( about )
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order - Deletion (Promotor Driven Cassette) VG11658 ZEN-UB1.GB view

Intermediate Vectors no data available