IKMC Project Report - KOMP-Regeneron (ID: VG14908)

Wnt5b MGI:98959 ENSMUSG00000030170 OTTMUSG00000027047
Pre-pipeline Designs Vectors ES Cells Mice
Germline Transmission Achieved

Mice

Microinjection Status Allele Allele Type ES Cell Clone Genetic Background QC Data MI/Distribution Centre
currently unavailable Genotype confirmed Wnt5btm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) 14908A-B4 C57BL/6NCrl;C57BL/6NCrl;C57BL/6N view
( about ) 		UCD/
Southern Blot na TV Backbone Assay na 5' LR-PCR na
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) na
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR na LoxP Confirmation na 3' LR-PCR
Genotyping Comment -
currently unavailable Genotype confirmed Wnt5btm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) 14908A-B4 C57BL/6NCrl;B6N-Tyrc/BrdCrCrl;C57BL/6N view
( about ) 		TCP/
Southern Blot na TV Backbone Assay na 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) na
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation na 3' LR-PCR
Genotyping Comment -

Mutagenesis Predictions no data available

ES Cell Clones Without Conditional Potential (Deletions)

Note: Mutations of type "Deletion" are correctly targeted clones that have had the target exon removed. These mutations cannot be converted into conditional alleles.

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Tools: Southern Blot Tool
ES Cell Clone Targeting Vector Allele Allele Type Parental ES Cell Line Genbank File Mouse QC Data
order 14908A-B4 VG14908 Wnt5btm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view yes no data reported    ( about )
order 14908A-D10 VG14908 Wnt5btm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view no no data reported    ( about )
order 14908A-G10 VG14908 Wnt5btm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view no no data reported    ( about )
order 14908A-G3 VG14908 Wnt5btm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view no no data reported    ( about )
order 14908A-H2 VG14908 Wnt5btm1(KOMP)Vlcg Deletion (Promotor Driven Cassette) VGB6 view no no data reported    ( about )
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - UCSC (mouse)
Design ID Vector Type Targeting Vector Cassette Backbone Genbank File
order - Deletion (Promotor Driven Cassette) VG14908 ZEN-Ub1 view

Intermediate Vectors no data available