<pre>LOCUS       KO-first_condition_ready_42025_MGI:2148742        21927 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Cldn16 targeting project(s): 42025
ACCESSION   unknown
SOURCE      Mus musculus.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_hubi_P
COMMENT     design_id: 46517
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..8977
                     /note="5 arm"
     misc_feature    9005..9029
                     /note="B1 Gateway"
     misc_feature    9042..9089
                     /note="Frt"
     intron          9090..9964
                     /note="mouse En2 intron"
     misc_feature    9962..9964
                     /note="Splice Acceptor"
     exon            9965..9966
                     /note="mouse En2 exon"
     misc_feature    10178..10738
                     /note="ECMV IRES"
     gene            10749..13795
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13830..14085
                     /note="SV40 polyadenylation site"
     misc_feature    14089..14122
                     /note="loxP"
     promoter        14126..15336
                     /note="human ubiquitin promoter"
     promoter        15337..15406
                     /note="EM7 promoter"
     gene            15419..16210
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16408..16651
                     /note="SV40 polyadenylation site"
     misc_feature    16668..16715
                     /note="Frt"
     misc_feature    16722..16755
                     /note="loxP"
     misc_feature    9979..10056
                     /note="FCHK std_gpr"
     misc_feature    16771..16791
                     /note="B2 Gateway"
     misc_feature    8978..16821
                     /note="Synthetic Cassette"
     exon            17176..17278
                     /db_xref="ENSEMBL:ENSMUSE00000267396"
                     /type="targeted"
                     /note="target exon 1 ENSMUSE00000267396"
     misc_feature    16822..17483
                     /note="Critical Region"
     misc_feature    17507..17540
                     /note="Downstream LoxP"
     misc_feature    17484..17563
                     /note="synthetic loxP region"
     exon            20479..20643
                     /db_xref="ENSEMBL:ENSMUSE00000267391"
                     /note="ENSMUSE00000267391"
     misc_feature    17564..21927
                     /note="3 arm"
BASE COUNT     5751 a   5036 c   5101 g   6039 t
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattttat tacttcaaaa gaaggctgtt catagtagaa tttatttcat
     4321 cctcataata ggttttctcc taggatttct atttgcctta tgctataatt cttccagaca
     4381 taaggtatgt attaaaatta ttatgtataa tatgagcact gattatttat ataccagctt
     4441 ggggtccagt ttgattttaa ggcttttagc agacatttga gactgtgagt gtactcctat
     4501 ttgtctcaag acttcctact gcactgaggt ccacgttaca ggagctgagg gtgcatttgg
     4561 ccccatgagc tcatgaagaa ggtgctacag gattttccat tttgttgaaa gaatgaacaa
     4621 aagatgatcc atcaagattc acattaaacc ctgaataaga agtatgtcta tagtgaaata
     4681 aatgaccatc ttttaaaagg cttgatctca tacttgctat aatataaaga attaatacat
     4741 ccatttggat cattttttaa taggaaaaaa aattaagaaa aatatctaat cccctaaagt
     4801 gtttaataaa gctggtggga aaagtgtact tggcctaaat ggtgaggctt ttctggtttg
     4861 ctctttaaag agaacatttt tataaagtaa ggaaaaggca tctaattgtt attgagatac
     4921 aatctgtgac ctctgacatg ggtcagttag ccttacagac tatgatgcta tgctttctga
     4981 ggacagcatt gtcatgcagg caacaagaga attatagcaa aatctaagtg tttacatggt
     5041 ttctgcagtg taagtcaagt catttagtga caggagttct ggtggccggt gctatagaag
     5101 acaaaagcct taagggacaa ctgctttgag attccaaata gtcaatccca ggattaagta
     5161 ctagtttatg acactatagc tcaccaggaa gagtcttcat tgacataatt tctaagggat
     5221 tttggagtga taccaggtca gtataaaccc tccattctga gtacagtcac tccagctcat
     5281 ggatttaact gttgtagttt gtttattctg ctcacaagca catatacacc caggcattgt
     5341 ggtctatctt tgtaattcca gagcttatga atttgagatg tgaggattac tgcaagttct
     5401 agggggagtt agggctactg gccctctgtt gtatctaatc aggagaaaaa aattaataac
     5461 tgaacatata gacatatgaa gcaaaaattc agtgtcatat tgaccacctt gtacttcaga
     5521 tcaaagcagg gttagttatg catttatgtg aattcacagg aatcaacaaa ctttgtctcc
     5581 aataagtaac tattgagcat ctgttgttta tcaggatgga ggatgatgga atccttgaag
     5641 cagggagcca tatctaccct gcttgccgct gcattcccag cattaattgg catgccggtt
     5701 agaacttgac aagcattcag tgacatagat tagaatgtaa cattgtagaa aaattcctgt
     5761 ggcatcacag attgaaggag ataaactcct gctagaaaga gcatagagag aggtcttatg
     5821 gaaaaaggaa tgtcttttaa gtgagacttg agttccaggt agatatttaa caagcggaga
     5881 acaaagaaca ttggttttag tacagacaag gttggaataa cccaaagacc tgaggaacca
     5941 ggtctgtgca tgtgtgaagt ggtctttgca ggggtagtgg ggacatgttg agaatattca
     6001 catcgagata caagcagcca ctcactgtgg ctgtttccat ccataaacag ctcaagaacc
     6061 aaagctgatc aagtggacaa gggaaaaaag gaagccaggt gtctggattg tctgtgatat
     6121 ctctccactt ggaatatcag cgagcagtca gaagatgtgt tgtgccttct gctttgttga
     6181 tgttttggaa attttcatct ctcttgtgtg ggaaatgatc cgtgtctcac tctttggaca
     6241 aatgacatca ctggagcaaa aatcccttga acattgcttg cctgtgttat tcatacaata
     6301 ccgattgcat gttaaaaaca aacaacagaa acctcaaaat tagaactttg tatttcaaat
     6361 ggctccacga gattaaaatt ttacagtttt tgccctttaa gaaaggttag ccacactatt
     6421 taagtactag ttagccttta aagatcctta ttttaagact tccaagaacg ttcattggct
     6481 tgccttgcaa aatagttcct aataagccat tttcccccca caggcagaga ggttaatcaa
     6541 aagcaataga atataacaca gagggctttc ctaaaatgat aacttaaaat ttttttaacc
     6601 ttgtagtttg ttcacaaagg cacctttaag ggtatctaca gtaagtgctg cattaaacac
     6661 agaagtttag ttaattacag ccactcttct cacgtatctt ggctgagtgt gaataccaag
     6721 cgatttaata gtgtgcccct gggcattaat acctaatgaa attggattcc ttgtttcctc
     6781 taatgacctc attgcttttc gaaatattgc catggcaggt aaatgatcaa tagccttgaa
     6841 attgacacca gcactgggcc atagctggat atccttgtta ttaagaattg tgtgttatat
     6901 cgtgtatagt aggaatggtc acctataatg taagaagaat aagaaagcat gaaccatcat
     6961 tgtttgttcc accacgctaa ttaggataag ctggatttat gagctgagag actggggaca
     7021 tgggtactaa ctaatcctgc ttttttcccc catagtatag tgggtctgta tgtaccctaa
     7081 ctgtatcagt ttccataaca aatgacctgg agcaaattat tctctcattg tcctgaagac
     7141 cagaatttta agatcaaaat ctcttcaagg ttccattcag tgtctctgaa gctccaggaa
     7201 gaactgctct ggtacttcca gattctcgtt actgtgggtt gtccatgact tgtaaatgtg
     7261 tgactgccaa cacagaagtg gccctgtggc ctcatttcca tctgcctgtg ctttttcttt
     7321 gtaaaaatat gataaggaca ccatgtataa gtttagagtc tcttgtgcgg ctgatgtgat
     7381 gccctcttct caagcctgta actcagtagt atctacaggg ccatagtcac aggttctggg
     7441 gattaagatg ggcttgagtc ttttagaagg ctgtcattca tttcattatg tcctcatttt
     7501 tgccactgtc ttttttagtg aaaccctgta ctgccagtac ttaactcatg gtatttagaa
     7561 agttaccatc attttcattt gagctaacaa tttatactaa ttagcaattt ttaatatttg
     7621 aaaccaccca gtcattatct ttcttataca gatgaggaaa ttgaaattca aaacagggaa
     7681 gatcttgcca aaggttacta cacaactact aaaattgtca atctgtgttt taaaattata
     7741 ctcatatatg tgtatatatg tgcacatctt gctttgaaaa tcaatgtgtt aggttgtaca
     7801 agatggactt ttcctcatta gaattttgac ctctgtgttt tttggggtct gataatcttc
     7861 cataaaatca acattattat tttcagattt aacagtaggg acagcccgcc aaagtaatga
     7921 tttgaagaat gagagcctag gcgtgagaat caagttagga catcttcact gaaggttgtc
     7981 ttccaatatc caagtaccaa atacaggatg ccttagcctg aaagagagtt ctgaaatgag
     8041 ttttacgtgc acacaaacaa gacttgggca tacacacatg gatggaccag ttggagtctg
     8101 tatcctgcat tattattgac tgtgctgtta cagtattttt tttcttcctt atgcatctgt
     8161 ttccttgtct gtaaatagac gataacagct tgttaatgat ctttaaaaaa caaatgacat
     8221 aataacatac ttagatagct ggtacttcaa agtatagact caatataatt gttaatcatt
     8281 accaattgca aatattttac atacaaggca atgatgagag tatcaggaga gtttcagcaa
     8341 gccccaaata gaacaaagtg caagagtttc agagtgctca cgttactatg ttcacagtaa
     8401 tatgattttc ttttttgaaa agtttttctg tccatttact ccctctggac aatgtttcaa
     8461 catctctccc tttgcaactc tttcctgatc tactatctta tttattcttt gggaatgggg
     8521 ttttaaaatt gactttccac aataactgcg aaggttttct aagctaactc cagattttca
     8581 aggtaatgga atctgtaatt gagtctgacg aaataatgat attgcctctg ctccatattc
     8641 atgagctttg tcaagaatac ttgaaactat cgtctgcagg gatatttact actgttttta
     8701 aaaaggtttg tgctgactta ccacccacca ataaaggact agaagggtca ggacaccggt
     8761 ggtacctttt agcgctaagt ggatattatc acctgcatgc caattccaag taaacacgct
     8821 ttacaactgt ccaattctat tagattactt ctcagacctt tatgttcatg tcatagctta
     8881 aaggtttgta atatgtcagt atagcagaga gaaagacaat tggggatgct cagtcattta
     8941 ttgttgccct ttttcattta ttggttttct ttgctaaaag gcgcataacg ataccacgat
     9001 atcaacaagt ttgtacaaaa aagcaggctg gcgccggaac cgaagttcct attccgaagt
     9061 tcctattctc tagaaagtat aggaacttcg aaccctttcc cacaccaccc tccacacttg
     9121 ccccaaacac tgccaactat gtaggaggaa ggggttggga ctaacagaag aacccgttgt
     9181 ggggaagctg ttgggagggt cactttatgt tcttgcccaa ggtcagttgg gtggcctgct
     9241 tctgatgagg tggtcccaag gtctggggta gaaggtgaga gggacaggcc accaaggtca
     9301 gccccccccc cctatcccat aggagccagg tccctctcct ggacaggaag actgaagggg
     9361 agatgccaga gactcagtga agcctggggt accctattgg agtccttcaa ggaaacaaac
     9421 ttggcctcac caggcctcag ccttggctcc tcctgggaac tctactgccc ttgggatccc
     9481 cttgtagttg tgggttacat aggaaggggg acgggattcc ccttgactgg ctagcctact
     9541 cttttcttca gtcttctcca tctcctctca cctgtctctc gaccctttcc ctaggataga
     9601 cttggaaaaa gataagggga gaaaacaaat gcaaacgagg ccagaaagat tttggctggg
     9661 cattccttcc gctagctttt attgggatcc cctagtttgt gataggcctt ttagctacat
     9721 ctgccaatcc atctcatttt cacacacaca cacaccactt tccttctggt cagtgggcac
     9781 atgtccagcc tcaagtttat atcaccaccc ccaatgccca acacttgtat ggccttgggc
     9841 gggtcatccc cccccccacc cccagtatct gcaacctcaa gctagcttgg gtgcgttggt
     9901 tgtggataag tagctagact ccagcaacca gtaacctctg ccctttctcc tccatgacaa
     9961 ccaggtccca ggtcccgaaa accaaagaag aagaacccta acaaagagga caagcggcct
    10021 cgcacagcct tcactgctga gcagctccag aggctcaagg ctgagtttca gaccaacagg
    10081 tacctgacag agcagcggcg ccagagtctg gcacaggagc tcggtacccg gaagatctgg
    10141 actctagaga attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
    10201 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
    10261 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
    10321 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
    10381 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
    10441 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
    10501 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
    10561 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
    10621 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg
    10681 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa
    10741 ccatggaaga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
    10801 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    10861 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
    10921 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
    10981 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga
    11041 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
    11101 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
    11161 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
    11221 acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc
    11281 tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc
    11341 ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg
    11401 atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag
    11461 ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg
    11521 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg
    11581 gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg
    11641 aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg
    11701 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc
    11761 tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg
    11821 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact
    11881 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc
    11941 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
    12001 atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg
    12061 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc
    12121 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg
    12181 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg
    12241 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc
    12301 tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta
    12361 acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg
    12421 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc
    12481 cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga
    12541 acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc
    12601 agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt
    12661 tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg
    12721 caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg
    12781 aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga
    12841 acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg
    12901 aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg
    12961 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct
    13021 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt
    13081 tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
    13141 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc
    13201 agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc
    13261 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa
    13321 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
    13381 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
    13441 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
    13501 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
    13561 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
    13621 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
    13681 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
    13741 cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
    13801 aataataacc gggcaggggg gatctaagct ctagataagt aatgatcata atcagccata
    13861 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    13921 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    13981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    14041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggaat aacttcgtat
    14101 agcatacatt atacgaagtt atgtttggcc tccgcgccgg gttttggcgc ctcccgcggg
    14161 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
    14221 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
    14281 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
    14341 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
    14401 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
    14461 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
    14521 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
    14581 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
    14641 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
    14701 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
    14761 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
    14821 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
    14881 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
    14941 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
    15001 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
    15061 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
    15121 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
    15181 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
    15241 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
    15301 ccgctaaatt ctggccgttt ttggcttttt tgttagacgt gttgacaatt aatcatcggc
    15361 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg gatcggccat
    15421 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
    15481 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
    15541 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
    15601 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
    15661 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
    15721 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
    15781 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
    15841 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
    15901 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
    15961 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    16021 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
    16081 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    16141 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    16201 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
    16261 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    16321 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    16381 cccccggatc taagctctag ataagtaatg atcataatca gccatatcac atctgtagag
    16441 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    16501 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    16561 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    16621 ctcatcaatg tatcttatca tgtctggatc cgggggtacc gcgtcgagaa gttcctattc
    16681 cgaagttcct attctctaga aagtatagga acttcgtcga gataacttcg tatagcatac
    16741 attatacgaa gttatgtcga gatatctaga cccagctttc ttgtacaaag tggttgatat
    16801 ctctatagtc gcagtaggcg gttatttatg agtaaatgtt ggcacatgga agtgaaatag
    16861 attgtcttta tgttaaagca cgactaaacg acagaagaat atattcagtt ctggttttat
    16921 ccctctcagc catgatttat ttttccacgt gccacttcca atcaggaagt atatttattt
    16981 cttggcccag aaagctttta attgtcaatg tctaagggaa tatattctac tggcattgct
    17041 ttatataaat agacttctag ttatgcttgt aaccactgta ctttgataac aaacatacct
    17101 gagtgaccat ttgaccaaga ggaaatggag ttgcctttgc aaagtgtttc atacagtgtc
    17161 ttttccaatc tccaggtgag cactaaatgc agaggcctgt ggtgggagtg tgtaacaaac
    17221 gcttttgatg ggattcgaac ctgcgatgag tacgactcca tatatgcaga acatccctgt
    17281 atgtataatg taaacctttc tgtttcagtg tgccacggaa tactgagctc agatatccac
    17341 ttgttgtatg gtattaagga ttttaatgtg aaatattggc aattagaaat gggtatcatt
    17401 ttttaaatat gaatggaaac atattgtatc atatttcacc gttcctcctg attttgcaaa
    17461 ttcctttata gtaaaattat tttgagatgg cgcaacgcaa ttaatgataa cttcgtatag
    17521 catacattat acgaagttat ggtctgagct cgccatcagt tcattgtgat tttataatta
    17581 gaattgacaa gctacatgaa tagtactcat tgttgtgttt caactataac agctaaaaag
    17641 tagaaagttt aatacttttt tttcacttga agatttacta agatagagta gcagagagag
    17701 agagagagag agagagagaa tgaatatggg gaccatagat gaagacaaca gcacatgtga
    17761 ggaaccatta ctctcaatgc tggagacaat tccctcatct ttctctcttt tagctcttaa
    17821 tcctacctag atatcagcaa agccatgtgc ctataaataa agcaagcttt taaattaaca
    17881 atccattgtt aatgccctca accttcttcg cagcattgac atatttagaa ttttgaacca
    17941 tgccaagaaa taccagtcct gctattaaaa gaaaatgctt ttatgctttt caaagccagt
    18001 gatgggtgct agtgctacac agcatcaaga taaaagtggg tcctgtgcaa tgtacctccc
    18061 ccccccccca gtcatctatt ttagttcagg aaaacacatt taaagaaaac aatcttcaaa
    18121 acagcttcat ctggtatttg ggagttcagt gtctgagata atttttgcca aaatatataa
    18181 ggccttttaa ttacattttt ttgcttagaa atttttacaa cttcacattt tcctcactat
    18241 attcatctca ttttatacac acacacttac acacacacac aaaaggaaga agcatatgcc
    18301 tgcttctcac tctgttttaa aaatgtaacg tgtggaccaa gccagaagac aaactttatt
    18361 tattcatttt cttatttatt tatttattta tttatttatt tatttattta tttccatatt
    18421 tctgtgtgta catgtgtggg catgagcatg cccaggtgca gttttggagg tcagagtgtc
    18481 atttatttcc ttcttactgg gttctggggt ttgaactcgg atcttctggt ttgtgaacag
    18541 acctttgtgt gctgagtcat cttctgggtc caggaagtca agcaaaagtt aaagttttta
    18601 aattttaaat actggtatac acagtcatgg cagaagaatt agagccattc attagggaaa
    18661 caaatgttga tattttaata tgctcctgtt aaggatttac ctgtcctatc tcatatttga
    18721 ctgttttttt tacttattgc tttcaaatgg tatgtaactt tcatttgtat gcaaatatac
    18781 attcaaagac aacaataata aagatgtgtt ctagaaaaat aacttctcta catcctcttg
    18841 gttagaaggc tatctacaag gtgtagagat ttcattttat tttctactaa attcataaaa
    18901 tcaatgtaga tagattttac agtttctcct gtatgtggca cctgatagtt atttgtgctt
    18961 tggtgcatct tttgttaaaa atagtttgag caagcccaag taaggatgct aactctgttc
    19021 tggatgccag aattcaaccc ttgacagaag aaattcttcc atacgttgct tgagcatttt
    19081 gcttgtaaca gatctgccct cctggaacca tactgaacac ggggctcaaa tcctagagct
    19141 tcatagagaa cctccatatg gaacagaaca tgcaagactc tctataggaa gaggaaagca
    19201 gggccaatgt agatgtgcat gaatatctca tcctaacagc actaatgact ttgggttgtc
    19261 caaagtcttc tcattagtag agggcccact tatgtactga gtattgaatt aaagaaaaat
    19321 ttttaaaaat ctagcatata aaggttagct cttcataatt tttcttttag ttttttttga
    19381 aaatatatgt atccatttga tttctattag aaataatgac aatttttaag atagatattc
    19441 ctaggctcag gataaataca tactcatgca ttgataattg tcttggtttt ctgtcagagt
    19501 tgctgtgaga acatgttctg acaaataact gcagaggaac aaagctttct tttggctcag
    19561 agttaaagga ggcaggaaag tcaatgtagc agggacttga accagctgct caccctggga
    19621 aggagagaag ggtgaatgct tgtggccagt tcattttatc atttttaaat agcccatggc
    19681 ccaagaccag gaaacagtgc tacacacctt tagcatggga ttttccatct caattaattt
    19741 aactggtgaa tgcttcgcag gcatgcttag aagccaagca atcccttgga gatttgtcta
    19801 ggggctgtat cctttctata tcctgtcatt tggacaacca gtagtcatat cataataata
    19861 catgggcata tacagtgtat tcacctgatg ataagatagg aagaatttca tatagtaatt
    19921 tgttttcttc tgcaagtata tcatataaat cttaactttt taaacaggga aaatgcccta
    19981 tatttctcaa aaacataagc cagaatttta tctcctcatt gaaaaaaatg taaaggcaaa
    20041 aagtcccaaa ttattatgaa aataagacat aattagcata tatgaaatga aaatatatta
    20101 atatttttat gatctaggta atataaattg ttcatactct atttttgatg taattttctt
    20161 cctttttagt attttgaatc aaaccccaaa ccttcttctg tagaactctg tgtaactccc
    20221 actcagtttt gagtcacaac tacagttgct ttatgatcat tttgaatttc atgagaaatt
    20281 atagcccccc atattgaagc ttctatagat ataaattctg aattctatgt atggccttat
    20341 gttgctgtgt ctaatcttgt gttctgtgat gttcctgtgc aagttaatgt gtctgtacat
    20401 ctccccctcc tcctccttcc cacattcctc ctctgactcc tcttctccca cccctgctct
    20461 gactccctga tcttgcagtg aagctggtgg taactcgagc actgatgatc acagctgaca
    20521 ttttagctgg ctttggattc atcaccctgc tccttggtct ggactgtgtg aagttcctac
    20581 ctgatgaccc acaaattaaa gtccgccttt gctttgttgc agggaccaca ttactcattg
    20641 caggtactgg gcttggttta ttttattcaa taatcacgag tctctgatca tgttcgagtt
    20701 gtgctacacg aatttatcat ttctcctcat ttaaggcaat ctatccatac atatcttgcc
    20761 tcctgatagt gtacagctct tatatatacc agtgagttta tttaacaaac aatatatact
    20821 tgtttttaga aaagatcttg aagaccactg aatactcttt ttataaatca ataaacaaaa
    20881 atttaaatta ctgaagaaaa tccttgtggg tgttacaaaa aagatgagca aattaggtga
    20941 cttaagataa ctaaaggctg gaaccattat ctacaattag tctgccagac acagagtttt
    21001 aaagcatgca ttctgttttg tagttttttt taaaaaggtt tttccaaatt agttggtaaa
    21061 tgtaacaatg ttcaaaaaag ggatggtaac tgtcttagtc agggcttcta ttcccgcaca
    21121 aacatcatga ccaagaaaca agttggggag gaaagggttt attcagctta caattccata
    21181 ctgctgttta tcaccaagga agtcaggcct ggaactcaaa caggtcagga agcaggagct
    21241 gatgcagagg ccatggaggg atgttcttta ctggcttgcc tctcctggct tgctcagcct
    21301 gctctcttat agaaccaaaa ccaccagccc agagatggtc ccacccacaa gggcccgttc
    21361 ccccttgatc actaattgag aaaatgcctt acagctggat ctcatggagg catttcctca
    21421 actgaagctc ctttctctgt gataactcca gctgtgtcaa gttgacacaa aactagccag
    21481 tacagtaact tatatctttt tataatggtt acatgtttga atagcttaga tgaacaatta
    21541 gacttataaa tccaaataat gtttaaaata gatattacca aatctccagg agttgataat
    21601 atgcactgtc ccaccacgag aggataagta tttttcaaaa caccctctca acatatttca
    21661 ttgtctccca gttatctctt atcttaccta tgttatacaa ttttttactt aaaatagtgg
    21721 aaccaatgtg ctatgatttc aagtgagtca attattagag agggaaagct gtgggcttca
    21781 gtcaaattgt tgaaggaatg tcacttcttg acccatgctt acatggtccg gaacacgata
    21841 aaatgattgc tagaaaagat acagttttta catgggtgtt atcttcccct ccccctcccc
    21901 agcatactga attgcaatga attggac
//
</pre>