EUCOMM Gateway protocols

• High Throughput Gateway (96 Well) Reactions
• Three Way Gateway Reaction (In tubes)

EUCOMM Tissue Culture protocols

• Feeders for F6 Cells
• Protocol for Colony Picking
• Preparing DNA for Electroporation
• General Instructions for Single Cuvette Electroporation With DNA Targeting Vectors

N4 Protocols

• Medium and Reagents for JM8.N4 (C57/Bl6/N) ES Cells & Feeders
• Expanding JM8.N4 for Electroporation
• JN8.N4 Cells Electroporation Protocol
• Passaging/Splitting Cells

F6 Protocols

• Expanding JM8.F6 for Electroporation
• JM8.F6 Cells Electroporation Protocol
• Passaging/Splitting Cells

Genotyping Protocols

• ES Cell Genomic DNA Purification in 96 Well Format
• Exonuclease I/Phosphatase
• 384/96 Well Long Range Polymerase Chain Reaction (LRPCR)
• Sequencing Reaction Setup
• Genotyping protocol (Expand Long Template)

ICS Southern Blot protocol

Mouse Production Protocols

An overview of EUCOMM Mouse generation protocols

WTSI Mouse production protocol

• Chimera Mouse Production by Blastocyst Injection

CNR Mouse production protocol

• EUCOMM JM8 ES cell mouse production

ICS Mouse production protocol

• ICS Standard operating procedure for EUCOMM mice production