SR-PCR genotyping uses a combination of separate PCR reactions that detect the presence/absence of the cassette lacZ (confirms the presence of the cassette), the gene-specific wild type allele and the gene-specific mutant allele by short range PCR.
Interpretation of the consolidated results produces the genotype of the mouse:
· LacZ positive, mutant positive, wild type positive = heterozygous.
· LacZ positive, mutant positive, wild type negative= homozygous.
LacZ negative, mutant negative, wild type positive= wild type.