The 5’ cassette check used by WTSI confirms the presence of the upstream FRT site and that the 5' end of the cassette is still present. The assay is shown below.
| Assay |
5' Forward |
5' Forward Sequence |
3' Reverse |
3' Reverse Sequence |
Product Size |
| FRT |
5FRT_F |
AGGCGCATAACGATACCACGAT |
5FRT_R |
CCACAACGGGTTCTTCTGTT |
204bp |
