IKMC Project Report - EUCOMM (ID: 116778)

Adam3 MGI:102518 ENSMUSG00000031553 OTTMUSG00000035967
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 10 wild type transcripts, of which 4 are protein-coding. Following removal of the floxed region, 4 transcripts are predicted to produce a truncated protein product of which 2 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000033958 protein_coding No protein product (NMD) view

Predicted translation:

MLPLFLVLSYLGQVIAAGKDVETPLLQITVPEKIDTNIQDAKEAETQVIFTPTFWNIFA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000890663 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001080990 Peptidase_M12B_N (23/121 aa) CDS CDS
ENSMUSE00001002662 Peptidase_M12B_N (19/121 aa) CDS Deleted
ENSMUSE00001055017 Peptidase_M12B_N (27/121 aa) CDS CDS + stop codon + UTR
ENSMUSE00000986341 Peptidase_M12B_N (26/121 aa) CDS
ENSMUSE00001080956 Peptidase_M12B_N (28/121 aa) CDS
ENSMUSE00001027654 Peptidase_M12B (12/197 aa) CDS
ENSMUSE00000973973 Peptidase_M12B (24/197 aa) CDS
ENSMUSE00000990606 Peptidase_M12B (56/197 aa) CDS
ENSMUSE00000984804 Peptidase_M12B (28/197 aa) CDS
ENSMUSE00000998729 Peptidase_M12B (46/197 aa) CDS
ENSMUSE00001009464 Peptidase_M12B (33/197 aa) | Blood-coag_inhib_Disintegrin (10/76 aa) CDS
ENSMUSE00001046952 Blood-coag_inhib_Disintegrin (33/76 aa) CDS
ENSMUSE00001005552 ADAM_Cys-rich (29/110 aa) | Blood-coag_inhib_Disintegrin (33/76 aa) CDS
ENSMUSE00000982296 ADAM_Cys-rich (30/110 aa) CDS
ENSMUSE00001011678 ADAM_Cys-rich (53/110 aa) CDS
ENSMUSE00000993062 EGF_extracell (10/30 aa) CDS
ENSMUSE00001061925 EGF_extracell (20/30 aa) CDS
ENSMUSE00001006588 CDS
ENSMUSE00000963768 CDS
ENSMUSE00001026276 CDS + stop codon + UTR
ENSMUSE00000684842 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000171438 protein_coding No analysis available: incomplete transcript
ENSMUST00000171611 protein_coding No protein product (NMD) view

Predicted translation:

MLPLFLVLSYLGQVIAAGKDVETPLLQITVPEKIDTNIQDAKEAETQVIFTPTFWNIFA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00001000108 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001080990 Peptidase_M12B_N (23/121 aa) CDS CDS
ENSMUSE00001002662 Peptidase_M12B_N (19/121 aa) CDS Deleted
ENSMUSE00001055017 Peptidase_M12B_N (27/121 aa) CDS CDS + stop codon + UTR
ENSMUSE00000986341 Peptidase_M12B_N (26/121 aa) CDS
ENSMUSE00001080956 Peptidase_M12B_N (28/121 aa) CDS
ENSMUSE00001027654 Peptidase_M12B (12/165 aa) CDS
ENSMUSE00000973973 Peptidase_M12B (24/165 aa) CDS
ENSMUSE00000990606 Peptidase_M12B (56/165 aa) CDS
ENSMUSE00000984804 Peptidase_M12B (28/165 aa) CDS
ENSMUSE00000898355 Peptidase_M12B (46/165 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000167431 protein_coding Target region does not overlap transcript
ENSMUST00000170318 nonsense_mediated_decay No protein product (NMD) view

Predicted translation:

MLPLFLVLSYLGQVIAAGKDVETPLLQITVPEKIDTNIQDAKEAETQVIFTPTFWNIFA*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000915753 UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001080990 Peptidase_M12B_N (24/94 aa) CDS CDS
ENSMUSE00001002662 Peptidase_M12B_N (19/94 aa) CDS Deleted
ENSMUSE00001055017 Peptidase_M12B_N (27/94 aa) CDS CDS + stop codon + UTR
ENSMUSE00000916234 Peptidase_M12B_N (24/94 aa) CDS
ENSMUSE00001073164 Peptidase_M12B_N (2/94 aa) CDS + stop codon + UTR
ENSMUSE00000995921 UTR UTR
ENSMUSE00001059357 UTR UTR
ENSMUSE00001056288 UTR UTR
ENSMUSE00001036707 UTR UTR
ENSMUSE00000991872 UTR UTR
ENSMUSE00001003963 UTR UTR
ENSMUSE00000967475 UTR UTR
ENSMUSE00000994278 UTR UTR
ENSMUSE00001022006 UTR UTR
ENSMUSE00001035330 UTR UTR
ENSMUSE00001038463 UTR UTR
ENSMUSE00001083555 UTR UTR
ENSMUSE00001023758 UTR UTR
ENSMUSE00001075458 UTR UTR
ENSMUSE00001030471 UTR UTR
ENSMUSE00000897347 UTR UTR
Legend: in frame deleted frameshifted
ENSMUST00000169598 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000167703 nonsense_mediated_decay No analysis available: incomplete transcript
ENSMUST00000166986 processed_transcript No analysis available: incomplete transcript
ENSMUST00000170740 processed_transcript No analysis available: incomplete transcript
ENSMUST00000168662 processed_transcript No analysis available: incomplete transcript
show/hide more transcripts

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available