IKMC Project Report - EUCOMM (ID: 117349)

Ydjc MGI:1916351 ENSMUSG00000041774 OTTMUSG00000024793
Pre-pipeline Designs Vectors ES Cells Mice
Design Completed

Mice no data available

Mutagenesis Predictions

This gene has 6 wild type transcripts, of which 3 are protein-coding. Following removal of the floxed region, 3 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000069064 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation:

MAFPRVRLVVTADDFGYCPRRDEGIVEAFLAGTVTSVSLLVNGTAAESAAELAR

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000428046 Uncharacterised_UPF0249/HpnK (48/283 aa) UTR + start codon + CDS
ENSMUSE00001056515 Uncharacterised_UPF0249/HpnK (54/283 aa) CDS Deleted
ENSMUSE00001091811 Uncharacterised_UPF0249/HpnK (34/283 aa) CDS Deleted
ENSMUSE00000988842 Uncharacterised_UPF0249/HpnK (60/283 aa) CDS Deleted
ENSMUSE00000278084 Uncharacterised_UPF0249/HpnK (90/283 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000115706 protein_coding Residual N-terminal, residual C-terminal product view

Predicted translation:

MAFPRVRLVVTADDFGYCPRRDEGIVEAFLAGTVTSVSLLVNGTAAESAAELARRDSSWSVTTAECSEGFAEFCSAGILP
SLQTENQCDLGHTGGKGRSPESFLPCTLGIALEMPAVGKRWKAGSSVIERKPNRELQLI*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000703915 Uncharacterised_UPF0249/HpnK (48/200 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001056515 Uncharacterised_UPF0249/HpnK (54/200 aa) CDS Deleted
ENSMUSE00001091811 Uncharacterised_UPF0249/HpnK (34/200 aa) CDS Deleted
ENSMUSE00000988842 Uncharacterised_UPF0249/HpnK (60/200 aa) CDS Deleted
ENSMUSE00000703914 Uncharacterised_UPF0249/HpnK (7/200 aa) CDS CDS
ENSMUSE00000703913 CDS CDS
ENSMUSE00000513877 Uncharacterised_UPF0249/HpnK (1/200 aa) CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000115702 protein_coding Residual N-terminal, residual C-terminal product view

Predicted translation:

MAFPRVRLVVTADDFGYCPRRDEGIVEAFLAGTVTSVSLLVNGTAAESAAELARRDSSWSVTTAECSEGFAEFCSAGILP
SLQTENQCDLGHTGGKGRSPESFLPCTLGIALGMWSVLCFPRIAV*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000703909 UTR UTR
ENSMUSE00000703908 UTR UTR
ENSMUSE00000703906 UTR UTR
ENSMUSE00000703905 Uncharacterised_UPF0249/HpnK (48/200 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00001056515 Uncharacterised_UPF0249/HpnK (54/200 aa) CDS Deleted
ENSMUSE00001091811 Uncharacterised_UPF0249/HpnK (34/200 aa) CDS Deleted
ENSMUSE00000988842 Uncharacterised_UPF0249/HpnK (60/200 aa) CDS Deleted
ENSMUSE00000703904 Uncharacterised_UPF0249/HpnK (7/200 aa) CDS + stop codon + UTR CDS + stop codon + UTR
Legend: in frame deleted frameshifted
ENSMUST00000145198 retained_intron No analysis available: incomplete transcript
ENSMUST00000142576 retained_intron No analysis available: incomplete transcript
ENSMUST00000137267 retained_intron No analysis available: incomplete transcript
show/hide more transcripts

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available