IKMC Project Report - KOMP-CSD (ID: 36047)

Cks1b MGI:1889208 ENSMUSG00000028044 OTTMUSG00000022072
Pre-pipeline Designs Vectors ES Cells Mice
Vector - Initial Attempt Unsuccessful

Mice no data available

Mutagenesis Predictions

This gene has 3 wild type transcripts, of which 2 are protein-coding. Following removal of the floxed region, 2 transcripts are predicted to produce a truncated protein product of which 0 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000029679 protein_coding Residual N-terminal, unknown C-terminal view

Predicted translation: 

MSHKQIYYSDKYDDEEFEYR

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000175427 Cyclin-dep_kinase_reg-sub (15/69 aa) UTR + start codon + CDS UTR + start codon + CDS
ENSMUSE00000979342 Cyclin-dep_kinase_reg-sub (44/69 aa) CDS Deleted
ENSMUSE00000175425 Cyclin-dep_kinase_reg-sub (12/69 aa) CDS + stop codon + UTR Deleted
Legend: in frame deleted frameshifted
ENSMUST00000107422 protein_coding No protein product
ENSMUST00000145486 nonsense_mediated_decay No analysis available: incomplete transcript
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ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available