IKMC Project Report - EUCOMM (ID: 47147)

F9 MGI:88384 ENSMUSG00000031138 OTTMUSG00000018393
Pre-pipeline Designs Vectors ES Cells Mice
Vector Unsuccessful - Project Terminated

Mice no data available

Mutagenesis Predictions

This gene has 1 wild type transcripts, of which 1 are protein-coding. Following removal of the floxed region, 1 transcript is predicted to produce a truncated protein product of which 1 may be subject to non-sense mediated decay (NMD). The original allele for this mutation is of type ''. The table below shows the predicted structure of the gene transcripts after application of Flp and Cre (forming a 'Knockout-First, Post-Flp and Cre - Deletion, No Reporter' allele - more information on IKMC alleles can be found here). Click the 'view' button for each transcript to see the full prediction for that transcript.

Ensembl transcript id Ensembl Biotype Floxed transcript description Details
ENSMUST00000033477 protein_coding No protein product (NMD) view

Predicted translation:

MKHLNTVMAESPALITIFLLGYLLSTECAVFLDRENATKILTRPKRYNSGKLEEFVRGNLERECIEERCSFEEAREVFEN
TEKTMEISVNQILV*

Ensembl Exon ID Pfam Domains WildType Floxed
ENSMUSE00000206740 CDS CDS
ENSMUSE00000206742 GLA_domain (32/42 aa) CDS CDS
ENSMUSE00000206733 GLA_domain (9/42 aa) CDS Deleted
ENSMUSE00000312879 GLA_domain (2/42 aa) | EGF-like_dom (31/31 aa) CDS CDS + stop codon + UTR
ENSMUSE00000206754 CDS
ENSMUSE00000312862 Peptidase_S1_S6 (14/228 aa) CDS
ENSMUSE00000206741 Peptidase_S1_S6 (39/228 aa) CDS
ENSMUSE00000381568 Peptidase_S1_S6 (176/228 aa) CDS + stop codon + UTR
Legend: in frame deleted frameshifted

ES Cell Clones no data available

Targeting Vectors no data available

Intermediate Vectors no data available