IKMC Project Report - mirKO (ID: mirKO31213)

Mir212 MGI:2676888 ENSMUSG00000065590
Pre-pipeline Designs Vectors ES Cells Mice
ES Cells - Targeting Confirmed

Mice no data available

Mutagenesis Predictions no data available

ES Cell Clones Without Conditional Potential (Deletions)

Note: Mutations of type "Deletion" are correctly targeted clones that have had the target exon removed. These mutations cannot be converted into conditional alleles.

Genome Browsers: Ensembl (mouse) - Ensembl (human) - UCSC (mouse) - UCSC (human)
Tools: Southern Blot Tool - LRPCR Genotyping Primers
ES Cell Clone Targeting Vector Allele Allele Type Deleted Exon Parental ES Cell Line Genbank File Mouse QC Data
order from MMRRC
order from EUMMCR 		mirKO_ES_PuDtk_17A1 mirKO_TV_PuDtk_PL4B3 Deletion (Promotor Driven Cassette) JM8A3 view no view    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order from MMRRC
order from EUMMCR 		mirKO_ES_PuDtk_17A2 mirKO_TV_PuDtk_PL4B3 Deletion (Promotor Driven Cassette) JM8A3 view no view    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
order from MMRRC
order from EUMMCR 		mirKO_ES_PuDtk_17A3 mirKO_TV_PuDtk_PL4B3 Deletion (Promotor Driven Cassette) JM8A3 view no view    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
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Targeting Vectors

Genome Browsers: Ensembl (mouse) - Ensembl (human) - UCSC (mouse) - UCSC (human)
Tools: LRPCR Genotyping Primers
Design ID Vector Type Targeting Vector Deleted Exon Cassette Backbone Genbank File
order from MMRRC
order from EUMMCR 		210336 Deletion (Promotor Driven Cassette) mirKO_TV_PuDtk_PL4B3 PGK_EM7_PuDtk_bGHpA PL611 view

Intermediate Vectors no data available